南首创艾滋病毒检测新法 检测HIV成本仅1元


南首创艾滋病毒检测新法 检测HIV成本仅1元

 
 
 
 
 

云南首创艾滋病毒检测新法 检测HIV成本仅1元

2009年09月18日10:04  来源:《春城晚报》

 

  目前,国际上HIV-1新发感染的检测方法有很多种,但大多数方法复杂且价格昂贵。由云南省疾病预防控制中心李洪等人首创的微量明胶颗粒凝集HIV-1近期感染检测方法(PA-LS)却是一种简单、便宜且易于推广的HIV检测新方法。“用这种方法检测一份样本成本只需要一元钱。”李洪说。

  创 新

  新方法可判断感染时间

  据李洪介绍,目前国际上常用的HIV检测方法主要检测被测者是否感染艾滋病病毒,而不能检测出感染艾滋病病毒有多长时间。“但是这个方法可以检测出哪些是HIV-1近期感染者,哪些是长期感染者。”

  “初筛为HIV抗体阳性,经过WB(HIV感染确认方法)确认是HIV感染后,用PA-LS法能够判断出感染者是在半年内感染艾滋病病毒的,还是在半年以前就感染上了。”李洪说,艾滋病也是一种传染病,在一定时间、一定人群中进行艾滋病病毒感染检测,如果能较为清楚地区分哪些是长期感染,哪些是近期感染,就能计算出该人群HIV的新发感染率。

  “计算出新发感染率,就能够帮助掌握艾滋病传播的流行动向、流行速度和趋势变化,对评价和评估艾滋病防治措施、投入效果有非常重要的作用。”他说。

  成 本

  检测一份样本仅一元钱

  据李洪介绍,PA-LS是在明胶颗粒凝集方法(PA)的基础上建立的。PA是世界上公认的一种简单、方便检测诊断HIV感染的方法,价格也比较昂贵。

  经过研究发现,改良后的PA方法在节约费用近数十倍的情况下,仍能保持较高的敏感度和特异性。“课题组通过研发,把检测一份HIV感染样本的PA试剂经过改良,就能用于检测三四十个HIV-1近期感染样本,这样就大大降低了成本。”李洪说,目前国际上有20多种检测HIV-1近期感染的方法,检测一份样本的成本大约是40元至200元不等,“但PA-LS成本只需要一元。”

  操 作

  无需仪器可用肉眼判别

  “只要培训半天,HIV初筛实验室的工作人员都会做。”李洪说,目前国际上所使用的方法都比较复杂,微量明胶颗粒凝集法(PA-LS)则“不需要仪器设备,结果可以用肉眼判别,而且非常易于推广”。

  李洪进一步介绍,虽然已经通过了研发试验,但是,如果要广泛推广应用,还需要进行更多的现场研究,特别是与国际上公认的一些方法进行比较性研究。“预计明年能有结果,希望云南和其他省市能够广泛应用。”(记者 杨质高 实习生 高旖)
 

Yunnan's first detection of HIV HIV testing new law only costs 1 yuan

At 10:04 on September 18, 2009 Source: "Spring City Evening News"

 

At present, the international HIV-1 detection of new infections There are many ways, but most methods is complex and expensive. Yunnan Provincial Center for Disease Control and Prevention by Li Hong, who pioneered micro-gelatin particle agglutination HIV-1 infection in the recent detection methods (PA-LS) is a simple, inexpensive and easy to promote HIV-testing new methods. "In this way the cost of testing a sample of only a dollar." Li Hong said.

Innovation

The new method to determine time of infection

According to Li Hong, at present the international HIV detection method commonly used in the main test measured whether HIV infection, and can not detect HIV infection how long. "But this method can detect which are infected with HIV-1 recently and which are long-term infection."

"Screening for HIV antibody positive, after WB (HIV infection to confirm methods) identified as HIV infected, with the PA-LS method can determine who is infected with HIV within six months, or six months before the infected with. "Li Hong said, AIDS is an infectious disease, in a certain time, a certain population for HIV testing, if a clearer distinction between what is a long-term infection, which is a recent infection, we can calculate the population of new HIV hair infection rates.

"Calculate the emerging infections, can help control the spread of AIDS epidemic trends, prevalence and trends of changes in the speed of assessment and evaluation of AIDS prevention and control measures put into effect there is a very important role." He said.

Cost

Testing of a sample of only one dollar

According to Li Hong introduction, PA-LS is a gelatin particle agglutination method (PA) based on the established. PA is the world's recognized as a simple, convenient method of detection and diagnosis of HIV infection, the price is also more expensive.

After the study found that the PA method improved cost savings over the past few times the case can still maintain a relatively high sensitivity and specificity. "Task Force through R & D, the detection of HIV infection in a sample of the PA reagent improved, thirty or forty can be used to detect recent HIV-1 infection in a sample, thus greatly reducing costs." Li Hong said that the current international there are more than 20 kinds of detection of recent HIV-1 infection was used to determine the cost of a sample of about 40-200 yuan range, "but the PA-LS cost only a dollar."

Operation

No instrument can determine the naked eye

"As long as a half-day training, HIV screening laboratory staff would do it." Li Hong said that the current methods used by the international community are complex, micro-gelatin particle agglutination (PA-LS) then "do not need equipment, results can distinguish with the naked eye and is very easy to spread. "

Li Hong further information, although the R & D test has passed, but if you want to be widely used, but also the need for more field research, particularly with the internationally recognized some of the methods of comparative study. "It is envisaged the outcome of next year, hoping Yunnan and other provinces and cities can be employed widely." (Young's high quality and high fluttering intern)
 
 
 
 
 
 

[ 作者:佚名    转贴自:本站原创    点击数:200    更新时间:2009-9-18    文章录入:nnb ]

 

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